Sulfur reducing bacteria from coal pits waste heaps of Chervonograd mining region

Abstract

Sulfur reducing bacteria from coal pits waste heaps of Chervonograd minig region were isolated and their seasonal number changes were established. Sulfur reducing bacteria number increases during cold season both at the end of vegetation season, depending on gangue humidity as well as on substrate temperature. The forty sulfur reducing bacteria isolates were selected. According to the highest biomass acumulation and hydrogen sulfide production for the following identification two strains were chosen. The morpho-physiological characteristics of isolated strains SV 30 and SV 35 were investigated. In accordance with obtained data, we assumed isolated strain SV 30 to be identified as genus Desulfuromusa, meanwhile SV 35 – Geobacter. After the seventh day of cultivation, the highest sulfur reducing activity of both strains was observed. Due to the metabolization ability of wide range of pollutants isolated sulfur reducing bacteria are perspective for application in environmental remediation technologies with biological methods.

Keywords

• sulfur reducing bacteria
• Geobacter
• Desulfuromusa
• coal pits waste heaps