Determination of Aflatoxins in Milk by PS-MWCNT/OH Composite Nanofibers Solid-Phase Extraction Coupled with HPLC-FLD

Abstract

In this work, a sensitive analytical method based on packed-nanofiber solid-phase extraction (PFSPE), after derivatization with trichloroacetic acid and high-performance liquid chromatography with a fluorescence detector (HPLC-FLD), has been established for the determination of aflatoxins (AFs) in milk. Polystyrene polymeric multi-walled carbon nanotube (PS-MWCNT/OH) composite nanofibers were fabricated by electrospinning and used to prepare homemade extraction columns. The extraction efficiency of the HPLC-FLD analysis method was sufficiently investigated and validated. After the implementation of optimal conditions, all of the analytes were separated efficiently and the components of the milk matrix did not disturb the determination. The obtained linear ranges of the calibration curves were 0.2–20 ng/mL for AFTB1 and AFTG2, 0.1–10 ng/mL for AFTB2, and 0.4–40 ng/mL for AFTG1. The recoveries ranged between 80.22% and 96.21%. The relative standard deviations (RSDs) for the intra-day and inter-day results ranged from 2.81–6.43% to 3.42–7.75%, respectively. Generally, 11 mg of sorbent and 200 μL of elution solvent were used to directly extract all of the AFs from the milk matrix. Reported herein is the first utilization of PS-MWCNT/OH-PFSPE HPLC-FLD to simultaneously analyze the occurrence of aflatoxins in milk.

Keywords

• aflatoxins
• packed-nanofiber solid-phase extraction (PFSPE)
• sample preparation
• polystyrene polymeric multi-walled carbon nanotube (PS-MWCNT/OH)
• milk