Морфологія (Jan 2013)
Analysis of expression of immunohistochemical markers of cell proliferation, vascularization, basal hyperplasia, stromal degradation, CD3+ and CD68+ mononuclear infiltration and Langerhans cells depending on the form and course of psoriasis vulgaris
Abstract
Background. In recent years, the efforts of dermatologists and patomorphologists directed to identifying additional prognostically significant criteria that will allow determining the psoriasis course and effectiveness of the treatment. Objective. To study the expression levels of immunohistochemical markers of cell proliferation, vascularization, apoptosis, inflammation, depending on the form of psoriasis and severity of pathological processes. Methods. The study included 18 patients (9 men and 7 women) aged from 24 up to 58 years with a diagnosis of psoriasis (general group) in two forms - plaque and guttate. To identify the relationship between angiogenesis, inflammatory infiltration and violations of keratinocytes maturation morphological and immunohistochemical studies were performed; primary antibody to CD3, CD68, Ki-67, VEGF, CD34, P63, S100, and MMP-9 were used. Results. The obtained results allow determining the form, stage and severity of psoriasis usual at the time of patient appeal and biopsies of psoriatic skin lesions obtaining. The activity of inflammation in psoriasis could be determined by neutrophils, macrophages and proliferative activity of the basal layer. Subsequent persistence of the pathological process - by the presence of basal cell hyperplasia in the regressing stage, overexpression of markers MMP-9, VEGF and dilation of blood vessels with T-lymphocyte infiltrates. Conclusion. Exclusively morphological criteria can not be used as reliable indicators of patient’s recovery with plaque and guttate forms of psoriasis and require additional immunohistochemical investigations. These additional prognostic markers also will help to develop effective schemes for patient treatment.
