RNA binding of Hfq monomers promotes RelA-mediated hexamerization in a limiting Hfq environment

Pallabi Basu; Maya Elgrably-Weiss; Fouad Hassouna; Manoj Kumar; Reuven Wiener; Shoshy Altuvia

doi:10.1038/s41467-021-22553-x

Nature Communications (Apr 2021)

RNA binding of Hfq monomers promotes RelA-mediated hexamerization in a limiting Hfq environment

Pallabi Basu,
Maya Elgrably-Weiss,
Fouad Hassouna,
Manoj Kumar,
Reuven Wiener,
Shoshy Altuvia

Affiliations

Pallabi Basu: Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University, Hadassah Medical School
Maya Elgrably-Weiss: Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University, Hadassah Medical School
Fouad Hassouna: Department of Biochemistry and Molecular Biology, IMRIC, The Hebrew University, Hadassah Medical School
Manoj Kumar: Department of Biochemistry and Molecular Biology, IMRIC, The Hebrew University, Hadassah Medical School
Reuven Wiener: Department of Biochemistry and Molecular Biology, IMRIC, The Hebrew University, Hadassah Medical School
Shoshy Altuvia: Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University, Hadassah Medical School

DOI: https://doi.org/10.1038/s41467-021-22553-x
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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RelA stimulates RyhB small RNA–target mRNA interaction by promoting assembly of Hfq monomers into hexamers. Here the authors show that RelA-mediated Hfq hexamerization requires an initial binding of RNA to Hfq monomers.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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