<em>Ochrobactrum anthropi</em>, contaminants of <em>in vitro</em> culture of sugarcane cells and tissues

Abstract

The purpose of this paper was to report, for first time, the presence of Ochrobactrum anthropi as contaminant of in vitro cell and tissue culture of sugarcane. Bacteria contaminant of in vitro plants were isolated directly from visibly contaminated plant culture on Tryptone soy agar medium and some drops of sugarcane cell suspension contaminated were inoculated on tubes with 3ml of Tryptone Soy Broth (TSB). Isolated bacteria were examined microscopically (shape, motility) and some biochemical test (Gram´s stain (Hucker´s modification), oxidase, catalase, O/F (oxidation/fermentation), were performed. Additional tests, according to Bergey´s Manual of Determinative Bacteriology, were performed. Identification was complemented by fatty acid analysis. Different bacteria can be contaminating plant cell and tissue culture. However, references about the presence of Ochrobactrum anthropi as contaminant of in vitro culture of plant cell and tissue have not been reached. For controlling microbial contaminants, is necessary to identify it. Key words: bacterial contamination,cell suspension, identification, in vitro plants, Saccharum