Nature Communications (Mar 2018)
Expansion of functional personalized cells with specific transgene combinations
- Christoph Lipps,
- Franziska Klein,
- Tom Wahlicht,
- Virginia Seiffert,
- Milada Butueva,
- Jeannette Zauers,
- Theresa Truschel,
- Martin Luckner,
- Mario Köster,
- Roderick MacLeod,
- Jörn Pezoldt,
- Jochen Hühn,
- Qinggong Yuan,
- Peter Paul Müller,
- Henning Kempf,
- Robert Zweigerdt,
- Oliver Dittrich-Breiholz,
- Thomas Pufe,
- Rainer Beckmann,
- Wolf Drescher,
- Jose Riancho,
- Carolina Sañudo,
- Thomas Korff,
- Bertram Opalka,
- Vera Rebmann,
- Joachim R. Göthert,
- Paula M. Alves,
- Michael Ott,
- Roland Schucht,
- Hansjörg Hauser,
- Dagmar Wirth,
- Tobias May
Affiliations
- Christoph Lipps
- Model Systems for Infection and Immunity, HZI – Helmholtz Centre for Infection Research
- Franziska Klein
- Department of Gene Regulation and Differentiation, HZI – Helmholtz Centre for Infection Research
- Tom Wahlicht
- Model Systems for Infection and Immunity, HZI – Helmholtz Centre for Infection Research
- Virginia Seiffert
- Department of Gene Regulation and Differentiation, HZI – Helmholtz Centre for Infection Research
- Milada Butueva
- Model Systems for Infection and Immunity, HZI – Helmholtz Centre for Infection Research
- Jeannette Zauers
- InSCREENeX GmbH
- Theresa Truschel
- InSCREENeX GmbH
- Martin Luckner
- InSCREENeX GmbH
- Mario Köster
- Department of Gene Regulation and Differentiation, HZI – Helmholtz Centre for Infection Research
- Roderick MacLeod
- Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures
- Jörn Pezoldt
- Experimental Immunology, HZI – Helmholtz Centre for Infection Research
- Jochen Hühn
- Experimental Immunology, HZI – Helmholtz Centre for Infection Research
- Qinggong Yuan
- Department of Gastroenterology, Hepatology, Endocrinology, Hannover Medical School
- Peter Paul Müller
- Department of Gene Regulation and Differentiation, HZI – Helmholtz Centre for Infection Research
- Henning Kempf
- Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, MHH
- Robert Zweigerdt
- Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, MHH
- Oliver Dittrich-Breiholz
- Research Core Unit Genomics, Medical School Hannover
- Thomas Pufe
- Department of Anatomy and Cell Biology, RWTH Aachen University
- Rainer Beckmann
- Department of Anatomy and Cell Biology, RWTH Aachen University
- Wolf Drescher
- Department of Orthopaedics, Aachen University Hospital, RWTH Aachen University
- Jose Riancho
- Department of Internal Medicine, Hospital U.M. Valdecilla, University of Cantabria, IDIVAL
- Carolina Sañudo
- Department of Internal Medicine, Hospital U.M. Valdecilla, University of Cantabria, IDIVAL
- Thomas Korff
- Institute of Physiology and Pathophysiology, RG Blood Vessel Remodeling, University Heidelberg
- Bertram Opalka
- Department of Hematology, West German Cancer Center (WTZ), University Hospital Essen
- Vera Rebmann
- Institute for Transfusion Medicine, University Hospital Essen
- Joachim R. Göthert
- Department of Hematology, West German Cancer Center (WTZ), University Hospital Essen
- Paula M. Alves
- Instituto de Biologia Experimental e Tecnologica, Universidade Nova de Lisboa
- Michael Ott
- Department of Gastroenterology, Hepatology, Endocrinology, Hannover Medical School
- Roland Schucht
- InSCREENeX GmbH
- Hansjörg Hauser
- Department of Gene Regulation and Differentiation, HZI – Helmholtz Centre for Infection Research
- Dagmar Wirth
- Model Systems for Infection and Immunity, HZI – Helmholtz Centre for Infection Research
- Tobias May
- InSCREENeX GmbH
- DOI
- https://doi.org/10.1038/s41467-018-03408-4
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 12
Abstract
Personalised medicine requires cell cultures from defined genetic backgrounds, but providing sufficient numbers of cells is a challenge. Here the authors develop gene cocktails to expand primary cells from a variety of different tissues and species, and show that expanded endothelial and hepatic cells retain properties of the differentiated phenotype.
