Qualitative Identification of <em>Roseburia hominis</em> in Faeces Samples Obtained from Patients with Irritable Bowel Syndrome and Healthy Individuals

Abstract

Various products coded by genes recognized in microbiota are involved in many biochemical pathways in thehuman body. Bacteria composition in the gastrointestinal tract may be an important aspect of selected diseases’ pathogenesis, including irritable bowel syndrome (IBS). Traditional research methods based on classical microbiology, using selective media for bacterial growth, have proven to be ineffective. The use of genetic methods allows the identification of unidentified microbiota, including anaerobes. Roseburia hominis is a flagellated gut anaerobic commensal bacterium, producing short-fatty acids. The knowledge about the microbial components of the intestinal ecosystem is still very limited, including Roseburia hominis. This study aimed to identify Roseburia hominis in the faeces samples obtained from IBS patients and healthy individuals, using PCR techniques. The differences between studied groups were observed, and it was concluded that R. hominis may play a role in IBS etiology.

Keywords

• gut microbiome
• gut microbiota
• <i>Roseburia hominis</i>
• IBS