Nature Communications (Nov 2021)
An integrative proteomics method identifies a regulator of translation during stem cell maintenance and differentiation
- Pierre Sabatier,
- Christian M. Beusch,
- Amir A. Saei,
- Mike Aoun,
- Noah Moruzzi,
- Ana Coelho,
- Niels Leijten,
- Magnus Nordenskjöld,
- Patrick Micke,
- Diana Maltseva,
- Alexander G. Tonevitsky,
- Vincent Millischer,
- J. Carlos Villaescusa,
- Sandeep Kadekar,
- Massimiliano Gaetani,
- Kamilya Altynbekova,
- Alexander Kel,
- Per-Olof Berggren,
- Oscar Simonson,
- Karl-Henrik Grinnemo,
- Rikard Holmdahl,
- Sergey Rodin,
- Roman A. Zubarev
Affiliations
- Pierre Sabatier
- Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institute
- Christian M. Beusch
- Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institute
- Amir A. Saei
- Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institute
- Mike Aoun
- Division of Medical Inflammation Research, Department of Medical Biochemistry and Biophysics, Karolinska Institute
- Noah Moruzzi
- The Rolf Luft Research Center for Diabetes and Endocrinology, Department of Molecular Medicine and Surgery, Karolinska Institute
- Ana Coelho
- Division of Medical Inflammation Research, Department of Medical Biochemistry and Biophysics, Karolinska Institute
- Niels Leijten
- Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
- Magnus Nordenskjöld
- Center for Molecular Medicine, Karolinska University Hospital
- Patrick Micke
- Immunology, Genetics and Pathology, Rudbecklaboratoriet, Uppsala University
- Diana Maltseva
- Faculty of biology and biotechnology, National Research University Higher School of Economics
- Alexander G. Tonevitsky
- Faculty of biology and biotechnology, National Research University Higher School of Economics
- Vincent Millischer
- Department of Molecular Medicine and Surgery, Karolinska Institute
- J. Carlos Villaescusa
- Neurogenetic Unit, Department of Molecular Medicine and Surgery, Karolinska University Hospital
- Sandeep Kadekar
- Department of Surgical Sciences, Uppsala University
- Massimiliano Gaetani
- Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institute
- Kamilya Altynbekova
- geneXplain GmbH, Am Exer 19B
- Alexander Kel
- geneXplain GmbH, Am Exer 19B
- Per-Olof Berggren
- The Rolf Luft Research Center for Diabetes and Endocrinology, Department of Molecular Medicine and Surgery, Karolinska Institute
- Oscar Simonson
- Department of Surgical Sciences, Uppsala University
- Karl-Henrik Grinnemo
- Department of Surgical Sciences, Uppsala University
- Rikard Holmdahl
- Division of Medical Inflammation Research, Department of Medical Biochemistry and Biophysics, Karolinska Institute
- Sergey Rodin
- Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institute
- Roman A. Zubarev
- Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institute
- DOI
- https://doi.org/10.1038/s41467-021-26879-4
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 16
Abstract
To characterize molecular changes during cell type transitions, the authors develop a method to simultaneously measure protein expression and thermal stability changes. They apply this approach to study differences between human pluripotent stem cells, their progenies, parental and allogeneic cells.
