The Residue Chemistry Transformation Linked to the Fungi Keystone Taxa during Different Residue Tissues Incorporation into Mollisols in Northeast China

Abstract

Managing carbon input from crop straw in cropland ecosystems could increase soil organic carbon (SOC) sequestration to achieve C neutrality and mitigate climate change. The complexity of the chemical structures of crop residue largely affects SOC sequestration. Fungi communities play an important role in the degradation of crop residues. However, the relationship between the fungal community composition and the chemical structures of crop residues remains unclear and requires further investigation. Therefore, a 120-day incubation experiment was conducted in Mollisols in Northeast China to investigate the decomposition processes and dynamics of maize straw stem (ST), leaf (LE) and sheath (SH) residues using 13C-NMR spectroscopy. Additionally, the microbiomes associated with these residues were analyzed through high-throughput sequencing to explore their relationship. Our results showed that the alkyl C contents in all treatments exhibited increases ranging from 15.1% to 49.1%, while the O-alkyl C contents decreased, ranging from 0.02% to 11.2%, with the incubation time. The A/OA ratios of ST, LE and SH treatments were increased by 23.7%, 43.4% and 49.3% with incubation time, respectively. During the early stages of straw decomposition, Ascomycota dominated, and in the later stage, Basidiomycota were predominant. The class of Sordariomycetes played a key role in the chemistry transformation of straw tissues during decomposition. The keystone taxa abundances, Fusarium_kyushuense, and Striatibotrys_eucylindrospora, showed strong negative correlations with di-O-alkyl C and carbonyl-C content and positive correlations with the β-glucosidase and peroxidase enzyme activity, respectively. In conclusion, our study demonstrated that the keystone taxa play a significant role in regulating the chemical structures of straw tissues, providing a better understanding of the influence of residue quality on SOC sequestration.

Keywords

• ¹³C-NMR spectroscopy
• chemical structure
• straw tissues
• fungi keystone taxa
• extracellular enzyme activity