The role of lysosomal membrane stability, malondialdehyde levels and DNA damage as pollution biomarkers in evaluating biological cleaning products using Mytilus galloprovincialis

Abstract

The possible effects of wastewater treatment products on mussels (Mytilus galloprovincialis) were studied. Three common pollution biomarkers were used: neutral red retention assay, malondialdehyde contents and DNA damage through comet assay. Two groups of mussels were treated in the laboratory for 25 days with 20 and 40 %v/v of treated wastewater collected after chlorination, and the control mussels formed a third group. The results showed statistically significantly lower neutral red retention times, higher malondialdehyde contents and higher formation of single-stranded DNA fragments in the mussels exposed at both treated wastewater concentrations compared to the controls. HIGHLIGHTS Statistically significantly lower NRR times were found in exposed mussels compared to controls.; Statistically significantly higher MDA values were found in exposed mussels compared to controls.; Remarkable sensitivity of the haemocytes to the formation of single-stranded DNA fragments were observed in the mussels exposed at both biological sample purification samples compared to the control ones.;

Keywords

• biological cleaning
• comet assay
• mda
• nrr