Rapid characterization of chemical constituents in Radix Tetrastigma, a functional herbal mixture, before and after metabolism and their antioxidant/antiproliferative activities

Yong Sun; Yan Qin; Hongyan Li; Han Peng; Hongbing Chen; Hai-rong Xie; Zeyuan Deng

Journal of Functional Foods (Oct 2015)

Rapid characterization of chemical constituents in Radix Tetrastigma, a functional herbal mixture, before and after metabolism and their antioxidant/antiproliferative activities

Yong Sun,
Yan Qin,
Hongyan Li,
Han Peng,
Hongbing Chen,
Hai-rong Xie,
Zeyuan Deng

Affiliations

Yong Sun: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, Jiangxi 330047, China
Yan Qin: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, Jiangxi 330047, China
Hongyan Li: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, Jiangxi 330047, China; Corresponding author. State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, Jiangxi 330047, China. Tel.: +86 791 88314447 8226; fax:+86 791 88304402.
Han Peng: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, Jiangxi 330047, China
Hongbing Chen: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, Jiangxi 330047, China
Hai-rong Xie: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, Jiangxi 330047, China
Zeyuan Deng: State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, Jiangxi 330047, China; Institute for Advanced Study, University of Nanchang, Nanchang, Jiangxi 330031, China; Corresponding author. State Key Laboratory of Food Science and Technology, University of Nanchang, Nanchang, Jiangxi 330047, China. Tel.: +86 791 88304402; fax: +86 791 88304402.

Journal volume & issue: Vol. 18
pp. 300 – 318

Abstract

Read online

Radix Tetrastigma (RT), the roots of Tetrastigma hemsleyanum Diels et Gilg, is consumed as both functional food and traditional medicinal herb in China, but there is rare report on its bioactive components. In the present study, the total flavonoid contents (TFC) and antioxidant/antiproliferative activities of RT were evaluated, and the chemical constituents of RT and their metabolites of rat after oral administration were identified and quantified by UPLC-ESI-QTOF-MS/MS and UPLC-ESI-QqQ-MS/MS. The extract of RT exhibited high TFC and antioxidant/antiproliferative activities in which there were 58 compounds characterized or tentatively identified. Nine original compounds and 12 metabolites were detected in plasma, while 11 original compounds and 13 metabolites were observed in urine. Nine flavonoids were quantified and 5 of them (catechin, kaempferol-3-rutinoside, rutin, isoquercitrin and astragalin) were up to 71.46% of TFC; their metabolites were also found in the plasma and urine, which was suggested to be the major antioxidant/antiproliferative compounds.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

About the journal

Abstract

Keywords