Purification of astrocyte subtype based on a double reporter mice approach for downstream transcription profiling

Abstract

Summary: Characterizing the molecular signature of a cell subtype leads to a better understanding of cell diversity, as this molecular data can identify new cellular markers and offer insights about cell function. Here, we describe an efficient protocol to separate a subtype of astrocytes, the Olig2-AS, from other glial cells by using a double reporter mouse approach and to determine the transcriptome profile of the Olig2-AS from the postnatal spinal cord using RNA-sequencing analysis.For complete details on the use and execution of this protocol, please refer to Ohayon et al. (2021).

Keywords

• Sequence analysis
• Cell Biology
• Cell isolation
• Flow Cytometry/Mass Cytometry
• RNAseq
• Model Organisms