Development of magnet configurations for magnetic immunostaining

Miki Kaneko; Shinichi Chikaki; Sachiko Matsuda; Akihiro Kuwahata; Masayuki Namita; Itsuro Saito; Satoshi Sakamoto; Moriaki Kusakabe; Masaki Sekino

doi:10.1063/1.5007918

AIP Advances (May 2018)

Development of magnet configurations for magnetic immunostaining

Miki Kaneko,
Shinichi Chikaki,
Sachiko Matsuda,
Akihiro Kuwahata,
Masayuki Namita,
Itsuro Saito,
Satoshi Sakamoto,
Moriaki Kusakabe,
Masaki Sekino

Affiliations

Miki Kaneko: The University of Tokyo, Tokyo, Japan
Shinichi Chikaki: The University of Tokyo, Tokyo, Japan
Sachiko Matsuda: Keio University, Tokyo, Japan
Akihiro Kuwahata: The University of Tokyo, Tokyo, Japan
Masayuki Namita: The University of Tokyo, Tokyo, Japan
Itsuro Saito: iMed Japan Inc., Tokyo, Japan
Satoshi Sakamoto: Tokyo Institute of Technology, Kanagawa, Japan
Moriaki Kusakabe: The University of Tokyo, Tokyo, Japan
Masaki Sekino: The University of Tokyo, Tokyo, Japan

DOI: https://doi.org/10.1063/1.5007918
Journal volume & issue: Vol. 8, no. 5
pp. 056732 – 056732-6

Abstract

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Magnetic immunostaining using a magnet and antibody-labeled fluorescent ferrite (FF) beads is established as a rapid immunostaining. In this study, we proposed the novel configuration of magnets with the large magnetic field gradient and the strong magnetic force for magnetic immunostaining. To confirm the usefulness of the proposed magnet configuration, we performed numerical analysis of the magnetic characteristics of the proposed magnets, and the magnetic immunostaining with FF beads. It was revealed that the proposed magnets generated the strong magnetic force and promoted the immunoreaction rapidly.

Published in AIP Advances

ISSN: 2158-3226 (Online)
Publisher: AIP Publishing LLC
Country of publisher: United States
LCC subjects: Science: Physics
Website: http://aipadvances.aip.org/

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