Osteogenic Regenerative Ability of Hydroxyapatite and Tricalcium Phosphate (Osteon III) in Rabbits

Abstract

Aims: Bone loss beyond the body's ability to regenerate may occur from various causes. The conventional methods of bone repair commonly used, such as autografts and allografts have their shortcomings and drawbacks. So, this study aims to evaluate the ability of biphasic hydroxyapatite and tricalcium phosphate 0steonIII (60% hydroxyapatite /40% tricalcium phosphate) for osteogenic regeneration in a rabbit model. Materials and Methods: Eighteen domestic rabbits were used in the current study. For each rabbit and following intramuscular general anesthesia, the selected site of surgery over the right femur was shaved and cleaned with povidone-iodine. A small incision of about 1.5 cm was created over the femur bone near its head avoiding any trauma to muscle. Two holes of 2 mm in depth and diameter and 15 mm apart were created under copious irrigation with distilled water in the femur. Using a small plastic scoop about 0.0260 g of Osteon III was added directly to fill one hole while the second hole was left empty to be filled with blood. At completion, the wound was closed using sutures. Based on this, the animals were divided into 2 groups and sacrificed at different time intervals at 7 days, 14 days, and 28 days. At the end of the aforementioned time interval, radiographic images of the femoral bones were taken at standard alignment and distance from the X-ray source, and histological assessment was conducted. Results: The osteon III bone defects showed high radio-opacity indicating new bone formation and mineralization due to the osteoconduction properties. Conclusions: This study supports that Osteon III may have better application prospects for bone repair.

Keywords

• bone substitutes
• osteogenic regeneration
• osteon iii