Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast

Ulschan Scheler; Wolfgang Brandt; Andrea Porzel; Kathleen Rothe; David Manzano; Dragana Božić; Dimitra Papaefthimiou; Gerd Ulrich Balcke; Anja Henning; Swanhild Lohse; Sylvestre Marillonnet; Angelos K. Kanellis; Albert Ferrer; Alain Tissier

doi:10.1038/ncomms12942

Nature Communications (Oct 2016)

Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast

Ulschan Scheler,
Wolfgang Brandt,
Andrea Porzel,
Kathleen Rothe,
David Manzano,
Dragana Božić,
Dimitra Papaefthimiou,
Gerd Ulrich Balcke,
Anja Henning,
Swanhild Lohse,
Sylvestre Marillonnet,
Angelos K. Kanellis,
Albert Ferrer,
Alain Tissier

Affiliations

Ulschan Scheler: Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry
Wolfgang Brandt: Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry
Andrea Porzel: Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry
Kathleen Rothe: Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry
David Manzano: Program of Plant Metabolism and Metabolic Engineering, Centre for Research in Agricultural Genomics
Dragana Božić: Department of Pharmaceutical Sciences, Group of Biotechnology of Pharmaceutical Plants, Laboratory of Pharmacognosy, Aristotle University of Thessaloniki
Dimitra Papaefthimiou: Department of Pharmaceutical Sciences, Group of Biotechnology of Pharmaceutical Plants, Laboratory of Pharmacognosy, Aristotle University of Thessaloniki
Gerd Ulrich Balcke: Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry
Anja Henning: Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry
Swanhild Lohse: Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry
Sylvestre Marillonnet: Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry
Angelos K. Kanellis: Department of Pharmaceutical Sciences, Group of Biotechnology of Pharmaceutical Plants, Laboratory of Pharmacognosy, Aristotle University of Thessaloniki
Albert Ferrer: Program of Plant Metabolism and Metabolic Engineering, Centre for Research in Agricultural Genomics
Alain Tissier: Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry

DOI: https://doi.org/10.1038/ncomms12942
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 11

Abstract

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Diterpenes are plant products with high antioxidant properties and potential application as food additives and therapeutics. Here, the authors describe the complete biosynthetic pathway of carnosic acid and reconstruct it in yeast, opening the way to metabolic engineering of phenolic diterpenes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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