In vitro propagation in Syringa josickaea Jacq.

Abstract

The report deals with several aspects of micropropagation in Syringa josickaea Jacq. There were employed various types of meristems, i. e. apical, tip axillary internode axillary, as well as differing lengths of meristems (0.2; 0.4; 0.6; 1.0 mm). Woody Plant Medium (WPM) medium supplemented with AIA (0.25 ml/l), ANA (0.1 ml/l), sacharose (30 g/l), agar (7 g/l), of 4.5 PH was used. Also, the influence of meristem type and length on the neoplant rooting percentage was studied. It was found that the type of meristem influenced plantlet formation and rooting. Best results were recorded with apical meristems (48.4% rooted plantlets). There is a positive correlation between the length of explants and the percentage of neoformation (explants length increase from 0.2 to 1.0 mm leads to the multiplication of this percentage by 3.7 times).

Keywords

• propagation
• Syringa josichaea
• in vitro