Cancer Management and Research (Dec 2020)
LCN2 Mediated by IL-17 Affects the Proliferation, Migration, Invasion and Cell Cycle of Gastric Cancer Cells by Targeting SLPI
Abstract
Jing Xu,1,2 ShengXiang Lv,1,2 Wei Meng,3 Fang Zuo4 1Department of Gastroenterology, The First People’s Hospital of Lianyungang, Lianyungang, Jiangsu Province, 222000, People’s Republic of China; 2Department of Gastroenterology, First Affiliated Hospital of Kangda College of Nanjing Medical University, Lianyungang, Jiangsu Province 222000, People’s Republic of China; 3Department of Functional Examination, Jinan Central Hospital, Jinan, Shandong Province 250014, People’s Republic of China; 4Department of Gastroenterology, Jinan Central Hospital, Jinan, Shandong Province 250014, People’s Republic of ChinaCorrespondence: Fang ZuoDepartment of Gastroenterology, Jinan Central Hospital, Jinan, Shandong Province 250014, People’s Republic of ChinaTel +86 13370582853Email [email protected]: Gastric cancer occurred in China and even the whole East Asia with high incidence. The objective of this study was to investigate the role of IL-17 in gastric cancer cells mediated by LCN2 binding to SLPI.Methods: The expression of LCN2 and SPLI in gastric cancer cells and transfection effects were confirmed by RT-qPCR analysis. The proliferation, clone formation ability, invasion, migration, apoptosis, and cell cycle of gastric cancer cells were in turn detected by CCK-8 assay, clone formation assay, transwell assay, wound healing assay, and flow cytometry analysis. The combination between LCN2 and SLPI was determined by co-immunoprecipitation assay. The expression of Caspase-3, Bcl-2, cyclinB1, cyclinD1, MMP9, and SLPI in gastric cancer cells was detected by Western blot analysis.Results: LCN2 and SPLI exhibited the highest levels in AGS cells, and thus AGS cells were selected for the next experiments. Down-regulation of LCN2 suppressed the proliferation and clone formation ability of AGS cells treated with IL-17. IL-17 promoted the invasion and migration of AGS cells, which was partially reversed by the down-regulation of LCN2. Down-regulation of LCN2 mediated by IL-17 promoted apoptosis and suppressed the cell cycle of AGS cells.Discussion: Down-regulation of LCN2 mediated by IL-17 suppressed the proliferation and suppressed the migration and invasion and cell cycle of gastric cancer cells by targeting SLPI.Keywords: lipocalin-2, LCN2, interleukin-17, IL-17, gastric cancer cells, SLPI
