Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope

Aaron Blanchard; J. Dale Combs; Joshua M. Brockman; Anna V. Kellner; Roxanne Glazier; Hanquan Su; Rachel L. Bender; Alisina S. Bazrafshan; Wenchun Chen; M. Edward Quach; Renhao Li; Alexa L. Mattheyses; Khalid Salaita

doi:10.1038/s41467-021-24602-x

Nature Communications (Aug 2021)

Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope

Aaron Blanchard,
J. Dale Combs,
Joshua M. Brockman,
Anna V. Kellner,
Roxanne Glazier,
Hanquan Su,
Rachel L. Bender,
Alisina S. Bazrafshan,
Wenchun Chen,
M. Edward Quach,
Renhao Li,
Alexa L. Mattheyses,
Khalid Salaita

Affiliations

Aaron Blanchard: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
J. Dale Combs: Department of Chemistry, Emory University
Joshua M. Brockman: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
Anna V. Kellner: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
Roxanne Glazier: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University
Hanquan Su: Department of Chemistry, Emory University
Rachel L. Bender: Department of Chemistry, Emory University
Alisina S. Bazrafshan: Department of Chemistry, Emory University
Wenchun Chen: Aflac Cancer and Blood Disorders Center, Children’s Healthcare of Atlanta
M. Edward Quach: Aflac Cancer and Blood Disorders Center, Children’s Healthcare of Atlanta
Renhao Li: Aflac Cancer and Blood Disorders Center, Children’s Healthcare of Atlanta
Alexa L. Mattheyses: Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham
Khalid Salaita: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University

DOI: https://doi.org/10.1038/s41467-021-24602-x
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 15

Abstract

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The authors have recently developed molecular force microscopy (MFM) which uses fluorescence polarisation to measure cell-surface receptor force orientation. Here they show that structured illumination microscopes, which inherently use fluorescence polarisation, can be used for MFM in a turn-key manner.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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