BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa

Tongbing Su; Weihong Wang; Zheng Wang; Peirong Li; Xiaoyun Xin; Yangjun Yu; Deshuang Zhang; Xiuyun Zhao; Jiao Wang; Liling Sun; Guihua Jin; Fenglan Zhang; Shuancang Yu

Cell Reports (Aug 2023)

BrMYB108 confers resistance to Verticillium wilt by activating ROS generation in Brassica rapa

Tongbing Su,
Weihong Wang,
Zheng Wang,
Peirong Li,
Xiaoyun Xin,
Yangjun Yu,
Deshuang Zhang,
Xiuyun Zhao,
Jiao Wang,
Liling Sun,
Guihua Jin,
Fenglan Zhang,
Shuancang Yu

Affiliations

Tongbing Su: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China
Weihong Wang: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China
Zheng Wang: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China
Peirong Li: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China
Xiaoyun Xin: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China
Yangjun Yu: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China
Deshuang Zhang: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China
Xiuyun Zhao: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China
Jiao Wang: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China
Liling Sun: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China
Guihua Jin: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China
Fenglan Zhang: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China; Corresponding author
Shuancang Yu: State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China; Beijing Key Laboratory of Vegetable Germplasms Improvement, Beijing 100097, China; Key Laboratory of Biology and Genetics Improvement of Horticultural Crops (North China), Beijing 100097, China; Corresponding author

Journal volume & issue: Vol. 42, no. 8
p. 112938

Abstract

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Summary: Increasing plant resistance to Verticillium wilt (VW), which causes massive losses of Brassica rapa crops, is a challenge worldwide. However, few causal genes for VW resistance have been identified by forward genetic approaches, resulting in limited application in breeding. We combine a genome-wide association study in a natural population and quantitative trait locus mapping in an F2 population and identify that the MYB transcription factor BrMYB108 regulates plant resistance to VW. A 179 bp insertion in the BrMYB108 promoter alters its expression pattern during Verticillium longisporum (VL) infection. High BrMYB108 expression leads to high VL resistance, which is confirmed by disease resistance tests using BrMYB108 overexpression and loss-of-function mutants. Furthermore, we verify that BrMYB108 confers VL resistance by regulating reactive oxygen species (ROS) generation through binding to the promoters of respiratory burst oxidase genes (Rboh). A loss-of-function mutant of AtRbohF in Arabidopsis shows significant susceptibility to VL. Thus, BrMYB108 and its target ROS genes could be used as targets for genetic engineering for VL resistance of B. rapa.

CP: Plants

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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