Bacteria Biofilms in Purulent-Septic Infections

Abstract

The causative agents of many infectious diseases can exist in the form of biofilms. The aim of the work is to study of the frequency of occurrence and the degree of activity of biofilm formation of microorganisms isolated from different locus in purulent-septic infections.Materials and methods. Fifteen strains isolated from patients with purulent-septic infections were examined. Biofilms were determined by the ability to adsorption a crystalviolet to ethanol.Results. 73,3 ± 11,4 % strains had biofilms (including gram-negative bacteria – 69,2 ± 11,9 %; Staphylococcus – 100,0 %; p < 0,05).The degree of activity of formation of biofilm by gram-negative bacteria was higher than Staphylococcus (0,302 ± 0,04 и 0,134 ± 0,01 units of optical density; p < 0,01). The highest activity of formation of biofilm was detected in K. pneumoniae isolated from patients with sepsis. Strains from clinically important locus (blood, sputum, wound discharge, abdominal fluid) had biofilms in 75,0 %; from locus of monitoring – 66,7 %. The pathogens isolated from locus of the monitoring were characterized by an average degree of activity of biofilm formation (0,180–0,360 units of optical density). Strains from clinically important locus (blood and sputum from patients with sepsis) had a highdegree of biofilm formation (more than 0,360 units of optical density). Conclusion. In most cases, strains were characterized by the presence of biofilms and differed in degrees activity of biofilm formation depending on locus.

Keywords

• bacteria biofilms
• purulent-septic infections
• locus of monitoring
• clinically important locus