Hemoparasites effect on interferon-gamma and miRNA 125b expression in Bubalus bubalis

Abstract

This study aimed to determine and evaluate the effect of the g4467 G>A SNP on the expression profile of IFN-γ and miRNA 125b in dairy buffaloes (Bubalus bubalis) with and without hemoparasites. Molecular diagnosis was performed by Polymerase Chain Reaction (PCR) on 145 buffaloes for Babesia spp., Trypanosoma vivax, and Anaplasma marginale. All buffaloes were investigated for the presence or absence of the polymorphism and genotyped using the restriction enzyme. Real-time PCR (RT-qPCR) quantified the expression of IFN-γ and miRNA 125b. All buffaloes were negative for Babesia spp. and A. marginale, and only 12 were positive for T. vivax. The genotypes GG, GA, and AA were found in proportions of 3.4%, 2.1%, and 94.5%, respectively. The A allele was the most frequent (95.5%). The SNP showed deviation from Hardy-Weinberg equilibrium (HWE) (P<0.05) and a deficit of heterozygotes with FIS 0.759. All animals of the found genotypes expressed both genes, except for GG positive for T. vivax. IFN-γ expression was higher for GA and GG negative (P<0.05) and AA positive. However, miRNA 125b expression was lower for AA and GA positive and higher for AA, GA, and GG negative. AA-positive buffaloes for T. vivax may exhibit susceptibility due to higher IFN-γ expression and lower miRNA 125b. GG and GA-negative buffaloes exhibited higher expression in both, suggesting they have greater resistance to positive ones.

Keywords

• buffalo
• hemoparasites
• ifn-γ
• mirna 125b
• t. vivax