Dealing with different methods for <it>Kluyveromyces lactis</it> &bgr;-galactosidase purification

Abstract

Several micro-scale chromatography-based procedures for purification of the &bgr;-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme.

Keywords

• Kluyveromyces
• Glucosidases