Nature Communications (Sep 2021)

Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9

  • Daniel M. Sapozhnikov,
  • Moshe Szyf

DOI
https://doi.org/10.1038/s41467-021-25991-9
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 26

Abstract

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The causal relationship between DNA demethylation and gene expression regulation has not yet been fully resolved. Here the authors develop a nuclease-dead Cas9 (dCas9) and gRNA site-specific targeting approach to physically block DNA methylation at specific promoters to cause DNA demethylation in cells and tackle this question.