Frontiers in Microbiology (Feb 2023)

Analysis of microbial diversity in the feces of Arborophila rufipectus

  • Xiaoping Ma,
  • Junshu Li,
  • Benping Chen,
  • Xinni Li,
  • Zhenwen Ling,
  • Shenglin Feng,
  • Sanjie Cao,
  • Zhicai Zuo,
  • Junliang Deng,
  • Xiaobo Huang,
  • Dongjie Cai,
  • Yiping Wen,
  • Qin Zhao,
  • Ya Wang,
  • Zhijun Zhong,
  • Guangneng Peng,
  • Yaozhang Jiang,
  • Yu Gu

DOI
https://doi.org/10.3389/fmicb.2022.1075041
Journal volume & issue
Vol. 13

Abstract

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IntroductionIntestinal microbiota composition plays a crucial role in modulating the health of the host. This evaluation indicator is very sensitive and profoundly impacts the protection of endangered species. Currently, information on the gut microbiota of wild birds remains scarce. Therefore, this study aimed to describe the gut microbial community structure and potentially, the pathogen composition of wild Arborophila rufipectus.MethodsTo guarantee comprehensive data analysis, we collected fecal samples from wild A. rufipectus and Lophura nycthemera in their habitats for two quarters. The 16S rRNA gene was then sequenced using high-throughput sequencing technology to examine the intestinal core microbiota, microbial diversity, and potential pathogens with the aim of determining if the composition of the intestinal microflora varies seasonally.Results and DiscussionThe gut microbiota of A. rufipectus and L. nycthemera primarily comprised four phyla: Proteobacteria (45.98%), Firmicutes (35.65%), Bacteroidetes (11.77%), and Actinobacteria (3.48%), which accounted for 96.88% of the total microbial composition in all samples. At the genus level, core microorganisms were found, including Shigella (10.38%), Clostridium (6.16%), Pseudomonas (3.03%), and Rickettsiella (1.99%). In these genera, certain microbial species have been shown to be pathogenic. This study provides important indicators for analyzing the health status of A. rufipectus and formulating protective measures.

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