The effect of up-regulation of NLE1 gene expression on the invasion and migration of colon cancer cells and its mechanism

Abstract

Objectives: To explore the regulatory effects of up-regulation of NLE1 gene expression in vitro and the mechanism of colon cancer cell invasion and migration. Methods: The logarithmic growth phase human colonic fibroblasts CCD-18Co, colon cancer cells HT-29, and NLE1 interference group were transfected with NLE1 siRNA to interfere with expressing viral vectors and liposomes. The NLE1 negative control group was added with liposomes, and NLE1 was overexpressed. The expression changes of RNA interference on the target gene were analyzed. Results: The expression of NLE1 mRNA in colon cancer cells was significantly higher (P < 0.05). Likewise, the expression of NLE1 protein in colon cancer cells was significantly increased than in control colon cancer cell lines (p < 0.05). Compared with the NLE1 interference group and the NLE1 negative control group, the migration and invasion ability of colon cancer cells in the NLE1 overexpression group were significantly varied (P < 0.05)‘. Conclusions: Up-regulation of the NLE1 gene in colon cancer cells promotes cell migration and invasion of colon cancer cells and inhibits the apoptosis ability of colon cancer cells.

Keywords

• Colon cancer
• Cell lines
• NLE1 gene
• HT-29
• Ribosome
• Invasion