PLoS ONE (Jan 2019)

Modulation of long-chain Acyl-CoA synthetase on the development, lipid deposit and cryosurvival of in vitro produced bovine embryos.

  • Roniele Santana Valente,
  • Tamie Guibu de Almeida,
  • Mayra Fernanda Alves,
  • Janine de Camargo,
  • Andrea Cristina Basso,
  • Katia Roberta Anacleto Belaz,
  • Marcos Nogueira Eberlin,
  • Fernanda da Cruz Landim-Alvarenga,
  • Patricia Kubo Fontes,
  • Marcelo Fábio Gouveia Nogueira,
  • Mateus José Sudano

DOI
https://doi.org/10.1371/journal.pone.0220731
Journal volume & issue
Vol. 14, no. 8
p. e0220731

Abstract

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In this study, we evaluated the modulation effect of long-chain Acyl-CoA synthetase during early embryo development. Bovine embryos were cultured in four groups: positive modulation (ACS+) with GW3965 hydrochloride, negative modulation (ACS-) with Triacsin C, association of both modulators (ACS±), and control. Embryo development rates were not altered (P>0.05) by treatments. Embryonic cytoplasmic lipid content increased in ACS+ but reduced in ACS- compared to the control (P 0.05) between groups; however, an increased apoptotic cells percentage was found in ACS- compared to control. Twenty-four hours after warming, ACS+ and control grade I embryos presented the best hatching rates, whereas the ACS+ group equaled the hatching rates between their embryos of grades I, II and III 48 hours after warming. The relative abundance of transcripts for genes associated with lipid metabolism (ACSL3, ACSL6, ACAT1, SCD, and AUH), heatshock (HSP90AA1 and HSF1), oxidative stress (GPX4), and angiogenesis (VEGF), among other important genes for embryo development were affected by at least one of the treatments. The treatments were effective in modulating the level of transcripts for ACSL3 and the cytoplasmic lipid content. The ACS- was not effective in increasing embryonic cryosurvival, whereas ACS+ restored survival rates after vitrification of embryos with low quality, making them equivalent to embryos of excellent quality.