Fecal transplant from vaginally seeded infants decreases intraabdominal adiposity in mice

Sivaranjani Namasivayam; Curtis Tilves; Hua Ding; Shaoguang Wu; Jada C Domingue; Camilo Ruiz-Bedoya; Ankit Shah; Eric Bohrnsen; Benjamin Schwarz; Mickayla Bacorn; Qing Chen; Shira Levy; Maria Gloria Dominguez Bello; Sanjay K Jain; Cynthia L Sears; Noel T Mueller; Suchitra K Hourigan

doi:10.1080/19490976.2024.2353394

Gut Microbes (Dec 2024)

Fecal transplant from vaginally seeded infants decreases intraabdominal adiposity in mice

Sivaranjani Namasivayam,
Curtis Tilves,
Hua Ding,
Shaoguang Wu,
Jada C Domingue,
Camilo Ruiz-Bedoya,
Ankit Shah,
Eric Bohrnsen,
Benjamin Schwarz,
Mickayla Bacorn,
Qing Chen,
Shira Levy,
Maria Gloria Dominguez Bello,
Sanjay K Jain,
Cynthia L Sears,
Noel T Mueller,
Suchitra K Hourigan

Affiliations

Sivaranjani Namasivayam: Clinical Microbiome Unit (CMU), Laboratory of Host Immunity and Microbiome, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Curtis Tilves: Department of Epidemiology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA
Hua Ding: Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Shaoguang Wu: Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Jada C Domingue: Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Camilo Ruiz-Bedoya: Center for Infection and Inflammation Imaging Research, Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Ankit Shah: Inova Health System, Inova Women’s Hospital, Falls Church, VA, USA
Eric Bohrnsen: Research Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases; National Institutes of Health, Hamilton, MT, USA
Benjamin Schwarz: Research Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases; National Institutes of Health, Hamilton, MT, USA
Mickayla Bacorn: Clinical Microbiome Unit (CMU), Laboratory of Host Immunity and Microbiome, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Qing Chen: Clinical Microbiome Unit (CMU), Laboratory of Host Immunity and Microbiome, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Shira Levy: Clinical Microbiome Unit (CMU), Laboratory of Host Immunity and Microbiome, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Maria Gloria Dominguez Bello: Departments of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ, USA
Sanjay K Jain: Center for Infection and Inflammation Imaging Research, Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Cynthia L Sears: Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
Noel T Mueller: Department of Epidemiology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, USA
Suchitra K Hourigan: Clinical Microbiome Unit (CMU), Laboratory of Host Immunity and Microbiome, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

DOI: https://doi.org/10.1080/19490976.2024.2353394
Journal volume & issue: Vol. 16, no. 1

Abstract

Read online

Exposing C-section infants to the maternal vaginal microbiome, coined “vaginal seeding”, partially restores microbial colonization. However, whether vaginal seeding decreases metabolic disease risk is unknown. Therefore, we assessed the effect of vaginal seeding of human infants on adiposity in a murine model. Germ-free mice were colonized with transitional stool from human infants who received vaginal seeding or control (placebo) seeding in a double-blind randomized trial. There was a reduction in intraabdominal adipose tissue (IAAT) volume in male mice that received stool from vaginally seeded infants compared to control infants. Higher levels of isoleucine and lower levels of nucleic acid metabolites were observed in controls and correlated with increased IAAT. This suggests that early changes in the gut microbiome and metabolome caused by vaginal seeding have a positive impact on metabolic health.

Published in Gut Microbes

ISSN: 1949-0976 (Print); 1949-0984 (Online)
Publisher: Taylor & Francis Group
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the digestive system. Gastroenterology
Website: https://www.tandfonline.com/journals/kgmi

About the journal

Abstract

Keywords