Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology

Abstract

The use of a Neubauer chamber is a broadly employed method when cell suspensions need to be quantified. However, this technique may take a long time and needs trained personnel. Spectrophotometry has proved to be a rapid, simple and accurate method to estimate the concentration of spore suspensions of isolates of the genus Fusarium. In this work we present a linear formula to relate absorbance measurements at 530 nm with the number of microconidia/ml in a suspension.

Keywords

• Espectrofotometría
• Cuantificación de esporas
• Métodos de laboratorio