Einstein (São Paulo) ()

Transection of the hepatic parenchyma associated or not with the contralateral portal vein branch ligature and its effect in liver regeneration

  • Henrique de Aguiar Wiederkehr,
  • Julio Cesar Wiederkehr,
  • Luiz Martins Collaço,
  • Eros Luiz de Sousa,
  • Paolo Salvalaggio,
  • Caroline Aragão de Carvalho,
  • Barbara de Aguiar Wiederkehr,
  • Camila Aparecida Moraes Marques,
  • Francielle França da Rosa,
  • Felipe de Negreiros Nanni,
  • Taíse Fuchs

DOI
https://doi.org/10.1590/s1679-45082017ao3831
Journal volume & issue
Vol. 15, no. 2
pp. 178 – 185

Abstract

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ABSTRACT Objective To analyze the influence of portal vein ligation in hepatic regeneration by immunohistochemical criteria. Methods Ten pigs divided into two groups of five animals underwent hepatectomy in two stages, and the groups were differentiated by ligation or not of the left portal vein tributary, which is responsible for vascularization of the left lateral and medial lobes of the pig liver. Five days after the procedure, the animals underwent liver biopsies for further analysis of histological and immunohistochemical with marker Ki67. Results The group submitted to hepatectomy with vascular ligation showed an increase of approximately 4% of hepatocytes in regeneration status, as well as a greater presence of Kupffer and inflammatory cells as compared to control. Conclusion As a result of positive cell replication observed through the Ki67 marker, we can suspect that the ligation of a tributary of the portal vein associated with liver resection promoted a greater stimulus of liver regeneration when compared to liver resection alone.

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