Chinese Journal of Lung Cancer (Apr 2010)
Expression of hTERT mRNA in Anip973 and Anip973/NVB Cell Detected by Quantitative Real-time PCR
Abstract
Background and objective Human telomerase reverse transcriptase is the catalytic subunit of telomerase, and its activity is correlated with cell’s sensitivity to chemotherapy. The aim of this study is to investigate the differential expression of human telomerase reverse transcriptase (hTERT) mRNA in human lung adenocarcinoma cell line Anip973 and Anip973/NVB, and to observe the correlation between hTERT mRNA and drug-resistance. Methods The real-time fluorescence quantitative RT-PCR was used to detect the change of hTERT mRNA in human lung adenocarcinoma drug-resistant cell Anip973/NVB and parental cell Anip973 treated by NVB. Results In the control group, the expression of hTERT mRNA showed no significant difference between drug-resistant cell Anip973/NVB and parental cell Anip973. After been treated by NVB, the expression of hTERT mRNA in parental cell was significantly decreased (P 0.05). The down-regulated hTERT mRNA in Anip973 cell was higher than that in Anip973/NVB cell. Conclusion Telomerase correlates with the drug-resistant cell A973/NVB, and telomerase may be a new target for multi-drug resistant inversion.
