Identification of key pathways and genes that regulate cashmere development in cashmere goats mediated by exogenous melatonin

Zhihong Liu; Zhihong Liu; Zhihong Liu; Zhihong Liu; Zhichen Liu; Qing Mu; Meng Zhao; Ting Cai; Yuchun Xie; Cun Zhao; Qing Qin; Chongyan Zhang; Xiaolong Xu; Mingxi Lan; Yanjun Zhang; Rui Su; Zhiying Wang; Ruijun Wang; Zhixin Wang; Jinquan Li; Jinquan Li; Jinquan Li; Jinquan Li; Yanhong Zhao; Yanhong Zhao; Yanhong Zhao; Yanhong Zhao

doi:10.3389/fvets.2022.993773

Frontiers in Veterinary Science (Sep 2022)

Identification of key pathways and genes that regulate cashmere development in cashmere goats mediated by exogenous melatonin

Zhihong Liu,
Zhihong Liu,
Zhihong Liu,
Zhihong Liu,
Zhichen Liu,
Qing Mu,
Meng Zhao,
Ting Cai,
Yuchun Xie,
Cun Zhao,
Qing Qin,
Chongyan Zhang,
Xiaolong Xu,
Mingxi Lan,
Yanjun Zhang,
Rui Su,
Zhiying Wang,
Ruijun Wang,
Zhixin Wang,
Jinquan Li,
Jinquan Li,
Jinquan Li,
Jinquan Li,
Yanhong Zhao,
Yanhong Zhao,
Yanhong Zhao,
Yanhong Zhao

Affiliations

Zhihong Liu: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Zhihong Liu: Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China
Zhihong Liu: Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
Zhihong Liu: Goat Genetics and Breeding Engineering Technology Research Center, Inner Mongolia Agricultural University, Hohhot, China
Zhichen Liu: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Qing Mu: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Meng Zhao: Inner Mongolia Autonomous Region Agriculture and Animal Husbandry Technology Extension Center, Hohhot, China
Ting Cai: Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, China
Yuchun Xie: Hebei Normal University of Science and Technology, Qinhuangdao, China
Cun Zhao: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Qing Qin: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Chongyan Zhang: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Xiaolong Xu: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Mingxi Lan: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Yanjun Zhang: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Rui Su: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Zhiying Wang: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Ruijun Wang: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Zhixin Wang: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Jinquan Li: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Jinquan Li: Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China
Jinquan Li: Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
Jinquan Li: Goat Genetics and Breeding Engineering Technology Research Center, Inner Mongolia Agricultural University, Hohhot, China
Yanhong Zhao: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China
Yanhong Zhao: Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China
Yanhong Zhao: Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, China
Yanhong Zhao: Goat Genetics and Breeding Engineering Technology Research Center, Inner Mongolia Agricultural University, Hohhot, China

DOI: https://doi.org/10.3389/fvets.2022.993773
Journal volume & issue: Vol. 9

Abstract

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The growth of secondary hair follicles in cashmere goats follows a seasonal cycle. Melatonin can regulate the cycle of cashmere growth. In this study, melatonin was implanted into live cashmere goats. After skin samples were collected, transcriptome sequencing and histological section observation were performed, and weighted gene co-expression network analysis (WGCNA) was used to identify key genes and establish an interaction network. A total of 14 co-expression modules were defined by WGCNA, and combined with previous analysis results, it was found that the blue module was related to the cycle of cashmere growth after melatonin implantation. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that the first initiation of exogenous melatonin-mediated cashmere development was related mainly to the signaling pathway regulating stem cell pluripotency and to the Hippo, TGF-beta and MAPK signaling pathways. Via combined differential gene expression analyses, 6 hub genes were identified: PDGFRA, WNT5A, PPP2R1A, BMPR2, BMPR1A, and SMAD1. This study provides a foundation for further research on the mechanism by which melatonin regulates cashmere growth.

Published in Frontiers in Veterinary Science

ISSN: 2297-1769 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: https://www.frontiersin.org/journals/veterinary-science

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