PLoS ONE (Jan 2017)

Human adipose derived stem cells regress fibrosis in a chronic renal fibrotic model induced by adenine.

  • Juan José Rivera-Valdés,
  • Jesus García-Bañuelos,
  • Adriana Salazar-Montes,
  • Leonel García-Benavides,
  • Alfredo Dominguez-Rosales,
  • Juan Armendáriz-Borunda,
  • Ana Sandoval-Rodríguez

DOI
https://doi.org/10.1371/journal.pone.0187907
Journal volume & issue
Vol. 12, no. 12
p. e0187907

Abstract

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ADSCs transplantation had been shown in some experimental models of kidney damage that it improves kidney function and reduces fibrosis. In this study we evaluated the effect of human adipose tissue-derived stem cell (hADSC) therapy in a chronic kidney damage experimental model.A chronic kidney injury was induced by daily orogastric administration of adenine (100mg/kg) to male Wistar rats for 28 days. hADSCs were isolated, expanded and characterized before transplantation. hADSC administration was performed in a tail vein at a dose of 2 x106 cells/animal. Animals were sacrificed at 7 days post-treatment. The percentage of fibrotic tissue, serum and urine levels of urea, creatinine, total protein and renal mRNA of COL1A1, TGFB1, CTGF, ACTA2, IL6, IL10, TNF were analyzed.hADSCs treatment significantly reduces kidney fibrosis, improves urea and creatinine serum and urine levels, and diminishes COL1A1, TGFB1, CTGF, ACTA2 mRNA kidney levels.These results showed that cell therapy using hADSCs improves renal function and reduces fibrosis.