THE COMPARISON BETWEEN FOUR POTATO CULTIVARS MULTIPLE AXILLARY BUD MICROPROPAGATION SYSTEM EFFICIENCY

Abstract

The growth in vitro of several potato cultivars (Amelia, Christian, Nicoleta and Roclas) on media containing plant growth regulators has been studied with a view to accelerating micropropagation of slow-growing cultivars. Whilst 0,5 mg/l GA3+0.1 mg/l IAA substantially increased the height of plantlets of most cultivars, the combination of 0,1 mg/l GA3+0,1 mg/l IAA+ 1,5 mg/l BAP was more effective in increasing the number of shoots which could subsequently be cultured. Using PM medium, a specific medium designed for potato, slow-growing or recalcitrant cultivars, such as Amelia, could be multiplied more rapidly than on basic Murashige & Skoog medium. Cultivars studied showed wide variation in their response to the plant regulators, best results being obtained for the cultivar Nicoleta.

Keywords

• micropropagation
• axillary bud
• hormonal balance
• culture medium