Lessons learned from conventional animals: Encouragement to use specific-pathogen-free animals

Tsunetaro Morino; Shun Kikuchi; Taro Inagaki; Manabu Komori; Kazuhisa Yamamoto; Hiromi Kojima; Masayuki Yamato

Regenerative Therapy (Jun 2020)

Lessons learned from conventional animals: Encouragement to use specific-pathogen-free animals

Tsunetaro Morino,
Shun Kikuchi,
Taro Inagaki,
Manabu Komori,
Kazuhisa Yamamoto,
Hiromi Kojima,
Masayuki Yamato

Affiliations

Tsunetaro Morino: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan; Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan
Shun Kikuchi: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan; Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan
Taro Inagaki: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan
Manabu Komori: Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan
Kazuhisa Yamamoto: Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan
Hiromi Kojima: Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan
Masayuki Yamato: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan; Corresponding author. Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo, 162-8666, Japan.

Journal volume & issue: Vol. 14
pp. 296 – 298

Abstract

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Experimental rabbits provide evidence for translational research regarding the pathogenies or treatment of human diseases. We developed a novel method for regenerating the middle ear mucosa using autologous cultured nasal mucosal epithelial cell sheets, and evaluated the wound healing process in the middle ear mucosa of experimental rabbits. Nonetheless, vigilant microbiological monitoring of experimental animals is essential to effectively prevent a decline in their health conditions, which may affect the research results. We experimented with contamination of Pasteurella multocida in non-specific-pathogen-free (SPF) rabbits (without microbiological monitoring). Most non-SPF rabbits had otitis media, whereas SPF rabbits did not, which affected their results during the mucosal regeneration study. The contamination was resolved by changing the experimental design from using non-SPF rabbits to that using SPF rabbits. It is crucial to use the SPF animals for any surgical intervention studies.

Published in Regenerative Therapy

ISSN: 2352-3204 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Biology (General): Cytology
Website: http://www.journals.elsevier.com/regenerative-therapy/

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