Electrochemical Determination of Interaction between SARS-CoV-2 Spike Protein and Specific Antibodies

Maryia Drobysh; Viktorija Liustrovaite; Ausra Baradoke; Alma Rucinskiene; Almira Ramanaviciene; Vilma Ratautaite; Roman Viter; Chien-Fu Chen; Ieva Plikusiene; Urte Samukaite-Bubniene; Rimantas Slibinskas; Evaldas Ciplys; Martynas Simanavicius; Aurelija Zvirbliene; Indre Kucinskaite-Kodze; Arunas Ramanavicius

doi:10.3390/ijms23126768

International Journal of Molecular Sciences (Jun 2022)

Electrochemical Determination of Interaction between SARS-CoV-2 Spike Protein and Specific Antibodies

Maryia Drobysh,
Viktorija Liustrovaite,
Ausra Baradoke,
Alma Rucinskiene,
Almira Ramanaviciene,
Vilma Ratautaite,
Roman Viter,
Chien-Fu Chen,
Ieva Plikusiene,
Urte Samukaite-Bubniene,
Rimantas Slibinskas,
Evaldas Ciplys,
Martynas Simanavicius,
Aurelija Zvirbliene,
Indre Kucinskaite-Kodze,
Arunas Ramanavicius

Affiliations

Maryia Drobysh: NanoTechnas—Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania
Viktorija Liustrovaite: NanoTechnas—Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania
Ausra Baradoke: State Research Institute Center for Physical and Technological Sciences, LT-10257 Vilnius, Lithuania
Alma Rucinskiene: NanoTechnas—Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania
Almira Ramanaviciene: NanoTechnas—Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania
Vilma Ratautaite: NanoTechnas—Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania
Roman Viter: Institute of Atomic Physics and Spectroscopy, University of Latvia, LV-1004 Riga, Latvia
Chien-Fu Chen: Institute of Applied Mechanics, National Taiwan University, Taipei City 106, Taiwan
Ieva Plikusiene: NanoTechnas—Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania
Urte Samukaite-Bubniene: NanoTechnas—Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania
Rimantas Slibinskas: Institute of Biotechnology, Life Sciences Center, Vilnius University, LT-10257 Vilnius, Lithuania
Evaldas Ciplys: Institute of Biotechnology, Life Sciences Center, Vilnius University, LT-10257 Vilnius, Lithuania
Martynas Simanavicius: Institute of Biotechnology, Life Sciences Center, Vilnius University, LT-10257 Vilnius, Lithuania
Aurelija Zvirbliene: Institute of Biotechnology, Life Sciences Center, Vilnius University, LT-10257 Vilnius, Lithuania
Indre Kucinskaite-Kodze: Institute of Biotechnology, Life Sciences Center, Vilnius University, LT-10257 Vilnius, Lithuania
Arunas Ramanavicius: NanoTechnas—Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, 03225 Vilnius, Lithuania

DOI: https://doi.org/10.3390/ijms23126768
Journal volume & issue: Vol. 23, no. 12
p. 6768

Abstract

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The serologic diagnosis of coronavirus disease 2019 (COVID-19) and the evaluation of vaccination effectiveness are identified by the presence of antibodies specific to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this paper, we present the electrochemical-based biosensing technique for the detection of antibodies specific to the SARS-CoV-2 proteins. Recombinant SARS-CoV-2 spike proteins (rSpike) were immobilised on the surface of a gold electrode modified by a self-assembled monolayer (SAM). This modified electrode was used as a sensitive element for the detection of polyclonal mouse antibodies against the rSpike (anti-rSpike). Electrochemical impedance spectroscopy (EIS) was used to observe the formation of immunocomplexes while cyclic voltammetry (CV) was used for additional analysis of the surface modifications. It was revealed that the impedimetric method and the elaborate experimental conditions are appropriate for the further development of electrochemical biosensors for the serological diagnosis of COVID-19 and/or the confirmation of successful vaccination against SARS-CoV-2.

Published in International Journal of Molecular Sciences

ISSN: 1661-6596 (Print); 1422-0067 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General); Science: Chemistry
Website: http://www.mdpi.com/journal/ijms

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