Study of Wound Dressing Material Coated with Natural Extracts of Calotropis Gigantean, Eucalyptus Globulus and Buds of Syzygium Aromaticum Solution Enhanced with rhEGF (REGEN-DTM 60)

Abstract

In this work, the leave extracts of Calotropis gigantean, Eucalyptus globulus and buds of Syzygium aromaticum were prepared and coated on natural fibers based bamboo gauze fabric for wound dressing applications. The fabrics coated with different compositions of the natural extract were enhanced with rhEGF (REGEN-D™ 60). The morphology of developed samples was studied using SEM to know its pores sizes. Various mechanical properties have been studied. The antibacterial activity was studied using Gram positive and negative types of bacteria. The wound healing efficacy of the coated fabric samples were studied through in vivo method. The type of wound selected for the study was second degree wound. The dorsal surface of the rat’s skin was removed and second degree wound was created. Then the developed fabric samples were dressed over the wound surface and the wound healing was studied by wound closure rate in equal interval of days. A specified composition of natural extracts coated on bamboo gauze fabric enhanced with rhEGF (REGEN-D™ 60) exhibits good antibacterial property was confirmed from the formation of zone of inhibition in antibacterial test against Escherichia coli and a Staphylococcus aureus and appropriate wound closure rate on second degree wound through in vivo evaluation.

Keywords

• anti-bacterial
• bamboo fiber
• leaves
• natural extract
• wound dressing
• wound healing