The study of Antimicrobial and Anti-adhesive effect of ProbioticLactobacilli on Uropathogenic Escherichia coli (UPEC)

Abstract

Introduction: One of the most important factors in Urinary Tract Infection caused by Uropathogenic Escherichia coli, is the attachment of bacteria to the host cell surface. Thus, inhibition of bacterial attachment is the appropriate action to prevent infection. The aim of this study was to investigate the antimicrobial and especially anti adhesive characteristics of probiotic bacteria against Escherichia coli by using microbial techniques. Materials and methods: In this study two strains of Lactobacillus acidophilus PTCC 1643 and Lactobacillus casei PTCC 1608 were used .40 Uropathogenic Escherichia coli were collected from Semnan province hospitals.20 samples with the more capability of biofilm production were selected for microbial tests. To evaluate the antimicrobial activity of complete culture and supernatant of probiotic lactobacilli, modified double layer method and dilution of supernatant were used, respectively. The mechanism of co- aggregation of lactobacilli with pathogens was examined. The microtitre plate method was used to detect anti-adhesive activity of Lactobacilli supernatant. Results: The antimicrobial and anti-adhesive effects of probiotic lactobacilli on Uropathogenic Escherichia coli were confirmed in all tests. In this study, Lactobacillus casie with the growth inhibitory (42/7 mm) and anti-adhesive (46/7mm) effects were reported as a proper probiotic bacterium. Discussion and conclusion: According to the results, the probiotic lactobacilli have spectacular effects to prevent attachment, biofilm formation and pathogenicity of UPEC, so using them to prevent and treat Urinary tract infection is a practical, reasonable and acceptable method.

Keywords

• Uropathogenic Escherichia coli
• Probiotic lactobacilli
• Urinary tract infection
• Antimicrobial and Antiadhesive Effect