Expression of MMP-2 in residual VX2 liver tumor after transcatheter arterial embolization combined with portal venous embolization in an animal model

Ning Wei; Ziqiang Wu; Dong Lu; Jingkun Xiao; Chunze Zhou; Senlin Chu; Weifu Lv

Journal of Interventional Medicine (Dec 2020)

Expression of MMP-2 in residual VX2 liver tumor after transcatheter arterial embolization combined with portal venous embolization in an animal model

Ning Wei,
Ziqiang Wu,
Dong Lu,
Jingkun Xiao,
Chunze Zhou,
Senlin Chu,
Weifu Lv

Affiliations

Ning Wei: Department of Radiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China; Corresponding author.
Ziqiang Wu: Department of Medical Imaging Center, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230036, China
Dong Lu: Department of Radiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China
Jingkun Xiao: Department of Radiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China
Chunze Zhou: Department of Radiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China
Senlin Chu: Department of Radiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China
Weifu Lv: Department of Radiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China

Journal volume & issue: Vol. 3, no. 4
pp. 167 – 173

Abstract

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Objective: This study aimed to analyze the effects of transcatheter arterial embolization (TAE) combined with portal venous embolization (PVE) on the expression of MMP-2 in residual VX2 liver tumor tissues, liver function and non-embolic lobe regeneration. Methods: A total of 72 rabbits were randomly divided into Sham, TAE, PVE and TAE + PVE groups (n = 18/group). The tissue samples from each group were taken at 6 h, 3 days and 7 days after interventional operation, respectively. MMP-2 expression was detected by immunohistochemistry, Real-time PCR, and Western-blotting. The main indicators (such as AST, ATL, and TBIL) of liver function and the volume of non-embolized hepatic lobes were measured in each group after operation. One-way ANOVA and Kruskal-wallis method were used for statistical analysis. Results: The expression of MMP-2 mRNA and protein remained the highest in the Sham group, and the expression of MMP-2 mRNA and protein in TAE, PVE and TAE + PVE groups were successively increased, and the expression of MMP-2 in TAE + PVE group was always significantly higher than TAE group. The AST and ALT levels in each group on day 7 after operation showed a significant declination, and all groups have recovered to the preoperative baseline level and TBIL has a slight fluctuation in each group after operation with no statistical difference. On day 7 after operation, the increasing volume of non-embolized liver lobes in TAE + PVE group showed a more significant effect than those in PVE group, but there was no statistical significance (37.62 ± 1.54 ml VS 36.18 ± 1.15 ml, P = 0.881), and its volume was significantly higher than those in the sham group (27.03 ± 1.11 ml). Conclusion: TAE + PVE is considered to be an efficient and safe approach for treating rabbit VX2 liver transplantation tumor, but the expression of MMP-2 increased fastest after TAE + PVE, which might promote tumor cell invasion and metastasis.

Published in Journal of Interventional Medicine

ISSN: 2096-3602 (Print); 2590-0293 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Medicine
Website: https://www.keaipublishing.com/en/journals/journal-of-interventional-medicine/

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