Isolation of Ligninolytic Enzymes Producing Microbes from Textile Effluent Contaminated Soil

Abstract

Evaluation of some microbial species for their ability to produce ligninolytic enzymes was investigated using streak plate method. Ten (10) microbial strains were isolated from soil contaminated with textile effluents using the spread plate technique. Aspergillus terreus and Aspergillus niger showed higher expression for laccase with 8.0 mm diameter zones clearance. Bacillus licheniformis and Bacillus subtilis had the widest clearance zone (12.0 and 8.0 mm) respectively. Only Aspergillus flavus however had the potential to produce lignin peroxidase (with 10 mm zones of clearance) of all the fungi isolated in this study. Streptococcus faecalis, Trichoderma harzianum, Micrococcus luteus, and Aspergillus flavus had the widest clearance zones (6.0 mm) in the Manganese peroxidase screening medium.Some of the microbial species possessed multiple traits for the production of the various ligninolytic enzymes assayed at ≥8 mm diameter zone clearance. Therefore, the research main focus is to identify microorganisms that are potential producers ofligninolytic enzymesgiven that they can be used for industrial waste bioremediation.

Keywords

• ligninolytic enzyme,
• peroxidase,
• effluents,
• bioremediation,
• clearance zones