Isolation and degradation characteristics of bacteria in black and odorous water

Abstract

Bioremediation is one of the treatment technologies for the black-odorous water, and obtaining functional bacteria is the key step to its success. In this study, a number of highly efficient pollutant degrading strains were isolated from the sediment of black-odorous river, and were identified by phenotypic and phylogenetic analysis. The composite bacterial agent J1 was mixed by strains A1, A2, A5 and A7, with a volume ratio of 4:4:2:1. And the final compound bacteria injected into black-odorous water were composed of J1 and NS3, with the volume ratio of 1:1. Optimized degradation conditions of compound bacterium agent were as follows: pH 7.5, DO 2.5 mg/L, temperature 30 °C. Under optimized conditions, add 1% by volume to the black-odorous water for a 50-day experimental operation. Finally, the overlying water ammonia nitrogen, chemical oxygen demand (COD), and total phosphorus has been significantly degraded. The research is expected to contribute to the use of bioremediation methods to repair black-odorous water, and the application of isolates can be carried out in situ for water types similar to pollute waterways. HIGHLIGHTS Controllable functional microbes for characteristic pollutants were obtained.; Physiological, biochemical and degradation characteristics of microbial isolates were explored.; A basic framework of in-situ bioremediation of black-odorous water was demonstrated.;

Keywords

• biochemical method
• black-odorous water
• compound bacterium agent
• in-situ remediation