Nature Communications (Jul 2020)
Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis
- Yong Zi Tan,
- José Rodrigues,
- James E. Keener,
- Ruixiang Blake Zheng,
- Richard Brunton,
- Brian Kloss,
- Sabrina I. Giacometti,
- Ana L. Rosário,
- Lei Zhang,
- Michael Niederweis,
- Oliver B. Clarke,
- Todd L. Lowary,
- Michael T. Marty,
- Margarida Archer,
- Clinton S. Potter,
- Bridget Carragher,
- Filippo Mancia
Affiliations
- Yong Zi Tan
- Department of Physiology and Cellular Biophysics, Columbia University
- José Rodrigues
- Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA)
- James E. Keener
- Department of Chemistry and Biochemistry, University of Arizona
- Ruixiang Blake Zheng
- Department of Chemistry, University of Alberta
- Richard Brunton
- Department of Chemistry, University of Alberta
- Brian Kloss
- Center on Membrane Protein Production and Analysis, New York Structural Biology Center
- Sabrina I. Giacometti
- Department of Physiology and Cellular Biophysics, Columbia University
- Ana L. Rosário
- Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA)
- Lei Zhang
- Department of Microbiology, University of Alabama at Birmingham
- Michael Niederweis
- Department of Microbiology, University of Alabama at Birmingham
- Oliver B. Clarke
- Department of Physiology and Cellular Biophysics, Columbia University
- Todd L. Lowary
- Department of Chemistry, University of Alberta
- Michael T. Marty
- Department of Chemistry and Biochemistry, University of Arizona
- Margarida Archer
- Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA)
- Clinton S. Potter
- National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center
- Bridget Carragher
- National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center
- Filippo Mancia
- Department of Physiology and Cellular Biophysics, Columbia University
- DOI
- https://doi.org/10.1038/s41467-020-17202-8
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 10
Abstract
The cryo-EM structure of Mycobacterium smegmatis arabinosyltransferase B EmbB involved in mycobacterial cell wall biosynthesis provides insights into the substrate binding and reaction mechanism. Mapping of the ethambutol resistance associated mutations onto the structure suggests the location of the drug binding site.
