A Comparative Transcriptome and Proteome Analysis in Rat Models Reveals Effects of Aging and Diabetes on Expression of Neuronal Genes

Qian Xu; Jing Cai; Yi-Bo Cong; Shao-Jian Xiao; Yun Liu; Wei Qin; Shi-Ya Chen; Hong Shi

doi:10.1016/j.ijge.2016.05.001

International Journal of Gerontology (Dec 2016)

A Comparative Transcriptome and Proteome Analysis in Rat Models Reveals Effects of Aging and Diabetes on Expression of Neuronal Genes

Qian Xu,
Jing Cai,
Yi-Bo Cong,
Shao-Jian Xiao,
Yun Liu,
Wei Qin,
Shi-Ya Chen,
Hong Shi

Affiliations

Qian Xu: Institute of Integrated Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, PR China
Jing Cai: Institute of Integrated Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, PR China
Yi-Bo Cong: Institute of Integrated Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, PR China
Shao-Jian Xiao: The Third People's Hospital of Fujian Province, Fuzhou, Fujian 350122, PR China
Yun Liu: Institute of Integrated Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, PR China
Wei Qin: Institute of Integrated Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, PR China
Shi-Ya Chen: Institute of Integrated Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, PR China
Hong Shi: Institute of Integrated Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, PR China

DOI: https://doi.org/10.1016/j.ijge.2016.05.001
Journal volume & issue: Vol. 10, no. 4
pp. 212 – 217

Abstract

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Background: To understand neuronal molecular changes in senile diabetes we established a rat senile diabetes model and analyzed transcriptome and proteome changes. Methods: Wistar rats were fed a high sugar, high fat diet for 16 months to induce diabetes. Non-diabetic aged rats and young rats were used as controls. Transcript and protein levels in the liver were then analyzed by microarray and antibody arrays, respectively. Results: Neuronal genes that were differentially expressed between senile diabetic rats, non-diabetic aged rats, and young rats were distributed across 12 pathways and 23 Gene Ontology (GO) clusters. Among them, 2267 genes were aging-related, 1230 genes were diabetes-associated, and 9 proteins might be associated with neurological disorders. Conclusion: In this study, we investigated transcriptome and proteome changes in animal models, analyzed the impact of aging and diabetes on neuronal molecules, and confirmed the correlations. Our study provides support for further studies on mechanisms of neuronal diseases.

Published in International Journal of Gerontology

ISSN: 1873-9598 (Print)
Publisher: Taiwan Society of Geriatric Emergency and Critical Medicine (TSGECM)
Country of publisher: Taiwan, Province of China
LCC subjects: Medicine: Internal medicine: Special situations and conditions: Geriatrics
Website: http://www.sgecm.org.tw/ijge/overview/

About the journal

Abstract

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