Oligomerization-primed coiled-coil domain interaction with Ubc13 confers processivity to TRAF6 ubiquitin ligase activity

Lin Hu; Jiafeng Xu; Xiaomei Xie; Yiwen Zhou; Panfeng Tao; Haidong Li; Xu Han; Chong Wang; Jian Liu; Pinglong Xu; Dante Neculai; Zongping Xia

doi:10.1038/s41467-017-01290-0

Nature Communications (Oct 2017)

Oligomerization-primed coiled-coil domain interaction with Ubc13 confers processivity to TRAF6 ubiquitin ligase activity

Lin Hu,
Jiafeng Xu,
Xiaomei Xie,
Yiwen Zhou,
Panfeng Tao,
Haidong Li,
Xu Han,
Chong Wang,
Jian Liu,
Pinglong Xu,
Dante Neculai,
Zongping Xia

Affiliations

Lin Hu: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University
Jiafeng Xu: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University
Xiaomei Xie: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University
Yiwen Zhou: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University
Panfeng Tao: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University
Haidong Li: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University
Xu Han: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University
Chong Wang: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University
Jian Liu: Department of Surgical Oncology, First Affiliated Hospital, School of Medicine, Zhejiang University
Pinglong Xu: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University
Dante Neculai: College of Medicine, Zhejiang University
Zongping Xia: Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University

DOI: https://doi.org/10.1038/s41467-017-01290-0
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 13

Abstract

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Ubiquitin ligase TRAF6 catalyzes assembly of free polyubiquitin chains for TAK1 activation in the IL-1R/TLR pathways, but the mechanism underlying its processivity is unclear. Here, the authors show that TRAF6 coiled-coil oligomerization domain primes its interaction with Ubc13/Ub~Ubc13 to confer processivity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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