The feasibility of an experimental model of donors after cardiac death in remote ischemic preconditioning studies for renal transplantation in porcine

Abstract

PURPOSE:To evaluate the feasibility of an experimental model of donors after cardiac death in remote ischemic preconditioning studies.METHODS: Twelve Landrace pigs were used as organ donors. They underwent cardiac arrest by coronary en block suture and interruption of ventilatory support. Haemodynamic data regarding the donor surgical protocol were evaluated. Studies variables included mean heart rate, systolic blood pressure, diastolic blood pressure, mean arterial pressure, central venous pressure and oxygen saturation and the time to death.RESULTS: Haemodynamic parameter indicated that the circulatory failure occurred after nine minutes of en blockcoronary suture and respiratory support interruption. The circulatory collapse occurred evenly across all groups. The heart rate and central venous pressure were statistically different between groups (p=0.023 and p=0.04), respectively. The remote preconditioning resulted in delayed time of death.CONCLUSIONS: The model is feasible, and was easily reproduced. The ischemic remote preconditioning tends to a slight increase in circulatory failure time.

Keywords

• Ischemic Preconditioning
• Kidney Transplantation
• Organ Preservation
• Swine