TESTING OF THE EFFICIENCY OF SOME ENZYMATIC MIXTURES, CONCERNING THE CONVERSION OF SEVERAL LIGNOCELLULOSIC BIOMASS’ SOURSES, TO REDUCING SUGARS

Abstract

Three lignocelluloses substrates have been used, as following: Mischantus, Maize stalk and Wheat bran, in order to obtain fermentescible sugars, which will be transformed into bioethanol. The substrates were hydrolysed using commercial enzymes: MethaPlus (β-glucanase, xylanase, cellulase), Veron 191 (xylanase), Hep C (cellulase). The hydrolysis was performed at 550 C, for 20 h, at pH = 5,5. The best results were obtained by using MethaPlus enzyme. The efficiency of hydrolysis was 110.80 % for Miscanthus, 126.15 % for maize stalk and 118.76 % for wheat bran, reported to the control. The most enhanced quantities of reducing sugars were obtained in maize stalk, namely: 126.15 % using MethaPlus, 112.07 % using Veron 191 and 113.52 % using Hep C. The wheat bran was hydrolysed with enzymatic mixtures, for emphasizing the reducing sugars’ grow, coming from residual starch’s content (flour). In comparison to the control, the efficiency of hydrolysis was of: 181.004 % for MethaPlus-Veron M4 mixture, 168.83 % for MethaPlus-Veron 393 mixture, 205.86 % for MethaPlus-BG α-malt mixture and of 176.57 % for MethaPlus-Veron MX mixture. The enzymatic mixture which contains BG α-malt was the most productive, the hydrolysis efficiency being superior to all other variants.

Keywords

• enzymatic mixtures
• enzymatic hydrolysis
• reducing sugars
• efficiency