Evaluation of patulin in commercial baby foods by solid phase extraction and liquid chromatography PDA detection

Abstract

A simple, reliable, and rapid RP-LC method has been developed for the determination of patulin in commercial baby foods from Turkish markets. Solid phase extraction and liquid chromatography with photodiode array detection technique were used for the analysis of patulin in samples. Linearity was obtained in different concentration ranges between 0.001 and 0.050 µg/l. The recovery rates for patulin ranged from 92.85% to 100.45% with a coefficient of variation less than 3.0%. LOD and LOQ of the method were found to be 9.66 × 10-6 and 2.93 × 10-5 µg/ml, respectively. The proposed method has been extensively validated in accordance with ICH guidelines. It can be used by routine analysis laboratories within a short time of analysis. All of the detected samples containing patulin were below the level suggested by the World Health Organization.

Keywords

• homogenised puree
• hplc
• patulin
• turkish markers
• spe