Coffee Pulp Activated Carbon for Immobilizing Cellulase from Aspergillus niger ICP2: Enhancing Enzyme Stability, Activity, and Its Reusability

Abstract

Cellulase from Aspergillus niger ICP2 was successfully immobilized on coffee pulp activated carbon using adsorption. Carbon was derived from coffee pulp via controlled carbonization at 200 °C for 2 hours, followed by activation with 3M ZnCl2 for 150 minutes. Reusability analysis exhibited over 50% relative activity retention after four cycles. The immobilized cellulase demonstrated stability at pH 4.0-6.0, retaining over 80% relative activity after 4 hours at 37℃. This approach proves economical for enhancing cellulase stability, activity, and reusability.