Journal of Clinical Medicine (Jul 2022)

Pancreas Preservation with a Neutrophil Elastase Inhibitor, Alvelestat, Contributes to Improvement of Porcine Islet Isolation and Transplantation

  • Ryusei Otsuka,
  • Chika Miyagi-Shiohira,
  • Kazuho Kuwae,
  • Kai Nishime,
  • Yoshihito Tamaki,
  • Tasuku Yonaha,
  • Mayuko Sakai-Yonaha,
  • Ikuo Yamasaki,
  • Misaki Shinzato,
  • Issei Saitoh,
  • Masami Watanabe,
  • Hirofumi Noguchi

DOI
https://doi.org/10.3390/jcm11154290
Journal volume & issue
Vol. 11, no. 15
p. 4290

Abstract

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For pancreatic islet transplantation, pancreas procurement, preservation, and islet isolation destroy cellular and non-cellular components and activate components such as resident neutrophils, which play an important role in the impairment of islet survival. It has been reported that inhibitors of neutrophil elastase (NE), such as sivelestat and α1-antitrypsin, could contribute to improvement of islet isolation and transplantation. In this study, we investigated whether pancreatic preservation with alvelestat, a novel NE inhibitor, improves porcine islet yield and function. Porcine pancreata were preserved with or without 5 μM alvelestat for 18 h, and islet isolation was performed. The islet yields before and after purification were significantly higher in the alvelestat (+) group than in the alvelestat (−) group. After islet transplantation into streptozotocin-induced diabetic mice, blood glucose levels reached the normoglycemic range in 55% and 5% of diabetic mice in the alvelestat (+) and alvelestat (−) groups, respectively. These results suggest that pancreas preservation with alvelestat improves islet yield and graft function and could thus serve as a novel clinical strategy for improving the outcome of islet transplantation.

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