Traditional and molecular diagnosis of Haemonchus contortus in sheep in Babylon province, Iraq

Abstract

Haemonchus contortus one of gastrointestinal helminthes characterized by miner intraspecific variation and a major variation among species which exploited to determine species simultaneously depend on PCR techniques, by amplification of DNA from parasites so goal of study was traditional and molecular detection of this parasite in sheep. Experiment was conducted using adult worms collected from abomasum of sheep obtained from abattoir in Babylon province. All worms divided into two groups, first group for morphological study and second group stored in 70% ethanol for DNA extraction, ITS-2 spacer and 28S gene were amplified using PCR technique sequenced and analysis with a phylogenic tree.According to the available data this study recordedHaemonchus contortus in sheep depend on ITS-2 spacer and 28S gene sequences for the first time in Iraq with accession no. LC552170 and LC552171 using molecular data.The phylogeny analysis depended on ITS-2 spacer and 28S gene partial sequences were closely related and high identity 94% with Germany H. similis sequence MN708992.1 and 93% identity with New-Zealand and Austria H. contortus sequence KC998713.1 and KJ724288.1 respectively, with a low genetic variation among all comparison sequenced isolates.

Keywords

• haemonchus contortus
• sheep
• its-2 spacer
• 28s genes