Investigations on a methodology of testing resistance to chemical stress of hop cultures in vitro

Abstract

The purpose of this research was to determine an adequate basic media in order to test the influence of some chemical stress factors (NaCl) on the resistance of hop neoplantules generated by minicuttings and cellular suspensions. The basic media was Murashige-Skoog (1962) with addition of phitohormones (auxins and cytokinins) and the chemical factor of stress was NaCl in three concentration (0,3%, 0,5% and 1%). At 1% NaCl we noticed an inhibition in growth. We selected the most vigorous plants, V1 and V2, which were relayered and passed on the same basic media. The operation was repeated several times in order to obtain forms highly resistant to salinity.

Keywords

• auxins
• neoplantules
• salinity
• cytokinins
• minicuttings
• basic media